Efficient surface-display of autotransporter proteins in cyanobacteria
نویسندگان
چکیده
منابع مشابه
Maximized Autotransporter-Mediated Expression (MATE) for Surface Display and Secretion of Recombinant Proteins in Escherichia coli.
A new optimized system for the surface display and secretion of recombinant proteins is described, termed MATE (maximized autotransporter-mediated expression). It is based on an artificial gene consisting of the coding region for the signal peptide of CtxB, a multiple cloning site for passenger gene insertion, flanked by coding sequences for linear epitopes for monoclonal antibodies and OmpT, a...
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Autotransporters (ATs) are a family of bacterial proteins containing a C-terminal β-barrel-forming domain that facilitates the translocation of N-terminal passenger domain whose functions range from adhesion to proteolysis. Genetic replacement of the native passenger domain with heterologous proteins is an attractive strategy not only for applications such as biocatalysis, live-cell vaccines, a...
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BACKGROUND Autotransporters represent a widespread family of secreted proteins in Gram-negative bacteria. Their seemingly easy secretion mechanism and modular structure make them interesting candidates for cell surface display of heterologous proteins. The most widely applied host organism for this purpose is Escherichia coli. Pseudomonas stutzeri A15 is an interesting candidate host for enviro...
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BACKGROUND Bacterial surface display technique enables the exogenous proteins or polypeptides displayed on the bacterial surface, while maintaining their relatively independent spatial structures and biological activities. The technique makes recombinant bacteria possess the expectant functions, subsequently, directly used for many applications. Many proteins could be used to achieve bacterial ...
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Bacterial pathogens must execute a prodigious array of complex functions in order to survive, multiply, and disseminate within mammalian hosts. Virulence determinants are usually proteinaceous in nature and are often either secreted to the bacterial cell surface or released into the external environment. Although secreted virulence proteins serve many divergent functions, remarkably, gram-negat...
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ژورنال
عنوان ژورنال: Algal Research
سال: 2015
ISSN: 2211-9264
DOI: 10.1016/j.algal.2015.09.013